Enhancement in biodegradability of distillery wastewater using enzymatic pretreatment.

A combined treatment technique consisting of enzymatic hydrolysis, followed by aerobic biological oxidation was investigated for the treatment of alcohol distillery spent wash. The enzyme cellulase was used for the pretreatment step with an intention of transforming the complex and large pollutant molecules into simpler biologically assimilable smaller molecules. Batch experiments were performed in order to analyze the influence of various parameters like pretreatment time, enzyme concentration and pH during the pretreatment step on the subsequent aerobic oxidation kinetics. The rate of aerobic oxidation was enhanced by 2.3 fold for the pretreated sample as compared to the untreated sample when the pH during the pretreatment step was maintained at a value of 4.8. Similarly, a two fold increase in the aerobic oxidation rate was found when the effluent was pretreated with the enzyme, without any pH control (i.e. effluent pH of 3,8). The study indicated that the enzymatic pretreatment of the effluent could be one of the successful pretreatments which can lead to enhancement of the rate of the subsequent aerobic oxidation.